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Rainbow probes

Universal DNA detection probes


With patent-pending universal Rainbow nucleic acid detection probes, no need to design and optimize a target-specific probe. We modify one of the primers of an existing pair (converting it into a Rainbow primer) so that it is recognized by a Rainbow probe during amplicon generation.

Advantages


No time lost for probe design and optimization

Available on stock with quick delivery

Maximally cost-efficient: same probe for different targets; no more unused stock in lab

Key features


Purposely developed for digital PCR

Highly concentrated (60X) for multiplexing

Compatible with ultrashort amplicons

Ideal for studies with high probe demands

Compose your universal Rainbow assay

Rainbow dPCR probes

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Rainbow qPCR probes

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Rainbow primer

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Detection principle and probe performance


Rainbow principle


We transform one of your existing PCR primers into a Rainbow primer with a 5' modification. This modified primer allows the Rainbow probe to detect the amplicon.

The Rainbow primer is used in combination with a regular primer and a universal Rainbow probe.

The Rainbow probe emits a bright signal when bound to the amplicon, but is dark when no target is present.






A Rainbow probe for every fluorophore


Compatible with all digital PCR Instruments. We have 13 fluorophores in stock for 6 different colors. 

The probes are 60X concentrated and stable for >30 days at room temperature.

   

    



Compatible with all dPCR instruments


All Rainbow probes have been successfully validated on most available digital PCR instruments, with excellent peak resolution.