Skip to Content

Human Sample ID kit

Track your samples effortlessly


The Human Sample ID Kit is an easy-to-use sequencing-based genotyping assay that targets polymorphic SNPs and gender markers, creating a highly specific intrinsic genetic label for each DNA sample. It comes in 2 versions, with either 49 or 13 markers. In a single reaction, short read sequencing-ready libraries are generated compatible. The Human Sample ID Kit is designed to be compatible with popular exome enrichment platforms and has been validated for both high-quality genomic, low-quality formalin-fixed paraffin-embedded (FFPE) and cell-free DNA templates.


Advantages


Excellent coverage uniformity

Easy integration in WES and WGS

Single reaction workflow

Analysis software with report

Key features


Extremely low random probability match of 1 in 27 quadrillion individuals

Ambient temperature shipment

Maximum compatibility with exome enrichment kits

Convenient single-use Unique Dual Index plates


Discover our solutions

Human Sample ID Kit

Your Dynamic Snippet will be displayed here... This message is displayed because you did not provide both a filter and a template to use.

Unique Dual Indexes plates

Your Dynamic Snippet will be displayed here... This message is displayed because you did not provide both a filter and a template to use.

Resources

Broc​hure

Manual
Software manual
SDS


Compatibility and workflow


Sample Authentication


Our convenient resequencing sample tracking solution is available in two versions. The Human Sample ID Kit targets 43 polymorphic SNPs and 6 gender markers, while the the smaller Human Sample ID mini kit targets 13 polymorphic SNPs. Each version creates a unique genetic label for every DNA sample.

 

Whole-exome sequencing (WES), whole-genome sequencing (WGS), and gene panel sequencing are routine practices in clinical genetic laboratories. The complex workflows, custody transfers, and large datasets impose data integrity challenges from the initial sample collection to the downstream data analysis. 

It has been estimated that sample mix-ups occur between 0.3% and 3% of the  cases, underscoring the need for an independent method for sample identity confirmation1. Several guidelines recommend interrogating SNPs via a second method to confirm patient identity (e.g., NPAAC 2017 Tier 4 S4.2).


 

The Human Sample ID Kit is designed to be compatible with established target enrichment platforms and has been validated for both high-quality genomic DNA, low-quality formalin-fixed paraffin-embedded (FFPE) tissue DNA, and cell-free DNA.  


Workflow 


In a single closed tube, sequencing-ready libraries are generated that are compatible with Illumina sequencing instruments. Our workflow offers a solution from sample preparation up to sequencing data analysis and sample tracking reporting.


The protocol consists of two reactions in the same (closed) tube: a multiplex PCR for the simultaneous amplification of 49 targets, and a (unique dual) indexing step for adding sample-specific barcodes. The one-step one-tube protocol drastically reduces hands-on time to 20 mins. 

After sample pooling and clean-up, the libraries are ready to be sequenced. The excellent coverage uniformity requires modest sequencing depth, making it a cost-effective assay. Data analysis and report generation are a breeze with our unique analysis software.

The Human Sample ID mini Kit uses the same workflow as the Human Sample ID Kit but targets 13 polymorphic SNPs, making this kit ideal for smaller gene panels that are compatible with these SNPs.